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Peptide Reconstitution & Dosage Calculator
Use our free, interactive Peptide Reconstitution & Dosage Calculator to determine accurate draw volume, liquid concentration (mg/mL and mcg/unit), and exact syringe tick marks for laboratory research. Compatible with U-100 (1mL), U-50 (0.5mL), and U-30 (0.3mL) insulin syringes.
Interactive Peptide Reconstitution Calculator
Accurate dilution math & visual syringe tick guide for laboratory investigations
mg
mL
Reconstitution Results
Draw Volume On Syringe
20 Units
(0.20 mL of solution)
Solution Concentration
2.50 mg/mL
Dose per 1 Unit Mark
25.0 mcg / unit
Doses per Vial
10 doses
Selected Syringe
U-100 (100 units)
0 Units
Fill: 20%
100 Units
Pull plunger down to the 20th unit line on your syringe barrel.
Standard Peptide Reconstitution Math Formula
Peptide reconstitution mathematics follows standard laboratory volumetric concentration formulas. The key relationship is:
Concentration (mcg/mL) = (Vial Quantity in mg × 1,000) ÷ Bacteriostatic Water Added (mL)
Draw Units on U-100 Syringe = (Desired Dose in mcg ÷ Concentration in mcg/mL) × 100
Common Peptide Reconstitution Reference Chart
| Peptide Name | Vial Quantity | Bac Water Added | Solution Concentration | Common Research Dose | Syringe Draw Mark |
|---|---|---|---|---|---|
| BPC-157 | 5 mg | 2.0 mL | 2.5 mg/mL (25 mcg/unit) | 250 mcg | 10 Units (0.10 mL) |
| TB-500 | 10 mg | 2.0 mL | 5.0 mg/mL (50 mcg/unit) | 2.5 mg (2,500 mcg) | 50 Units (0.50 mL) |
| GHK-Cu | 50 mg | 5.0 mL | 10.0 mg/mL (100 mcg/unit) | 2.0 mg (2,000 mcg) | 20 Units (0.20 mL) |
| Tirzepatide | 15 mg | 3.0 mL | 5.0 mg/mL (50 mcg/unit) | 2.5 mg | 50 Units (0.50 mL) |
| Retatrutide | 10 mg | 2.0 mL | 5.0 mg/mL (50 mcg/unit) | 2.0 mg | 40 Units (0.40 mL) |
| Semax | 10 mg | 2.0 mL | 5.0 mg/mL (50 mcg/unit) | 500 mcg | 10 Units (0.10 mL) |
Step-by-Step Laboratory Reconstitution Protocol
- Sterilization: Wipe the rubber septum of the peptide vial and the bacteriostatic water vial with a sterile 70% isopropyl alcohol prep pad. Allow 30 seconds for evaporative air drying.
- Draw Diluent: Using a sterile syringe, draw the exact calculated volume of bacteriostatic water (typically containing 0.9% benzyl alcohol as an antimicrobial preservative).
- Slow Side-Wall Inoculation: Insert the needle into the peptide vial at a 45-degree angle. Slowly trickle the water down the inside glass wall of the vial. Do not spray water directly onto the lyophilized peptide powder to prevent mechanical shear stress and denaturation.
- Gentle Swirling: Swirl the vial with a gentle circular wrist motion until the lyophilized pellet is completely dissolved into a clear solution. Never shake or vortex the vial.
- Refrigerated Storage: Store the reconstituted solution in a laboratory refrigerator at 2°C to 8°C (36°F to 46°F). Protect from UV light exposure. Most reconstituted peptides maintain stability for 21 to 28 days when preserved with bacteriostatic water.
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Frequently Asked Questions
Q. Why is bacteriostatic water preferred over sterile water?
Bacteriostatic water contains 0.9% benzyl alcohol, which acts as a bacteriostatic preservative that halts microbial proliferation. While sterile water is pure, it lacks preservatives; once punctured, it can support bacterial colonization within 24 hours. Bacteriostatic water allows multiple laboratory punctures for up to 28 days under refrigerated conditions.
Q. What is the difference between U-100, U-50, and U-30 syringes?
All standard U-100 insulin syringes share the same volumetric scale (100 units = 1.0 mL, meaning 1 unit = 0.01 mL). A U-100 syringe holds 1.0 mL (100 units), a U-50 holds 0.5 mL (50 units), and a U-30 holds 0.3 mL (30 units). Smaller syringes (U-30, U-50) offer wider barrel spacing, making micro-dose measurements significantly easier to read under laboratory conditions.
Q. Can I freeze reconstituted peptides?
Repeated freeze-thaw cycles can cause ice crystal formation that degrades peptide bonds and disrupts secondary structure. While lyophilized powder is safely stored at -20°C for years, reconstituted peptides should be kept refrigerated at 2°C to 8°C and used within their recommended experimental stability window.
Research-Use-Only Notice: This calculator and the associated content are provided strictly for educational, scientific, and in-vitro laboratory calculation purposes. Peptides supplied by PrecisionsPeptides.com are intended for laboratory research only and not for human or veterinary diagnostic or therapeutic use.